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eBioscience™ SAFE™ Human FGF basic, FGF-2, Recombinant Protein

Human Recombinant Protein

Brand:  Affymetrix eBioscience 68-8785-63

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15580627

  • £48.31 / 10µg


Estimated Shipment date
from Supplier 29-09-2017
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Description

Description

This SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.

Fibroblast Growth Factor basic (FGF-b, also known as FGF-2) is a member of the FGF family, a highly conserved family of 16-34 kDa heparin-binding proteins. FGF-b exists in several isoforms, and although they are equally active, only the 18 kDa form is secreted while the 23 kDa form localizes to the nucleus. It lacks the signal sequence peptide necessary for the ER/Golgi pathway, indicating that secretion occurs via an alternate pathway. FGF-b shares four common tyrosine kinase receptors, FGFR 1-4, and require the binding of a second surface protein, the ubiquitously expressed heparan sulfate proteoglycan, in order to fully activate these receptors. FGF family members affect the proliferation, differentiation, mobility, and survival of several cell types, including fibroblasts, osteoblasts, smooth muscle cells, and neuroblasts. FGF-b expression has been detected in several cell types, including fibroblasts, macrophages, endothelial cells, epithelial cells, and neurons. FGF-b is particularly important in embryonic development as triggers of neurogenesis, angiogenesis, and neovascularization and has most recently been studied for its ability to maintain the proliferation of embryonic stem cell cultures in an undifferentiated state. Some members of the family, including FGF-b, remain active during adulthood and play a role in bone formation and tissue repair. FGF family members are also implicated in many types of cancer and may contribute to tumor vascularization.
Specifications

Specifications

Functional Assays
17.8kDa
SDS-PAGE
10μg
The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile PBS to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Plant expressed 146 amino acids (Pro143-Ser288) with 10 amino acid N terminal tag- Accession NP_001997.5
The ED50 of this protein, as measured by proliferation assay of NIH3T3 mouse fibroblast cells, is less than or equal to 0.2ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.
Unlabeled
Yes
Sterile lyophilized (PBS buffer pH 7.2)
FGF basic, FGF-2
≥95%
Plant
RUO
Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
Recombinant Protein
Human