Invitrogen™ Human ATP5I (aa 41-69) Control Fragment Recombinant Protein

Product Code. 30210122

Brand: Invitrogen™ RP96729

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (84%), Rat (84%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-57112 (PA5-57112. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.

Specifications

• Accession Number: P56385
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 521
• Name: Human ATP5I (aa 41-69) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 2610008D24Rik; ATP synthase e chain, mitochondrial; ATP synthase membrane subunit e; ATP synthase subunit e, mitochondrial; ATP synthase subunit e, mitochondrial, N-terminally processed; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e; ATP synthase, H+ transporting, mitochondrial Fo complex subunit E; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E; Atp5i; Atp5k; Atp5me; ATPase subunit e; F1F0-ATP synthase, murine e subunit; Lfm1; Lfm-1
• Common Name: ATP5I
• Gene Symbol: Atp5me
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: RRIAAEEKKKQDELKRIARELAEDDSILK
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining