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Invitrogen™ Human ATE1 (aa 171-252) Control Fragment Recombinant Protein

Product Code. 30196527
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Quantity:
100 μL
Unit Size:
100µL
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30196527 100 μL 100µL
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Product Code. 30196527 Supplier Invitrogen™ Supplier No. RP97034

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Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (70%), Rat (70%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58391 (PA5-58391. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Arginyl-tRNA-protein transferase (ATE1), also designated arginyltransferase 1, belongs to the R-transferase family of proteins. In order for a protein to be degraded via the ubiquitin pathway, arginylation of the protein is required. ATE1 plays an important role in this process, as it is important for the posttranslational conjugation of arginine to the N-terminal aspartate-, glutamate- and possibly cystine-contaning substrates. ATE1 is a 518 amino acid protein. Alternative splicing results in two distinct isoforms. ATE1, which is found as a monomer, can localize to the cytoplasm and/or the nucleus.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O95260
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 11101
Name Human ATE1 (aa 171-252) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias AI225793; arginine-tRNA-protein transferase 1; arginine-tRNA--protein transferase 1; arginyltransferase 1; arginyl-tRNA-protein transferase; arginyl-tRNA--protein transferase 1; ATE1; AW547406; R-transferase 1
Common Name ATE1
Gene Symbol ATE1
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence DFVGEKLGSGEPSHSVKVHTVPKPGKGADLSKPPCRKAKEIRKERKRLKLMQQNPAGELEGFQAQGHPPSLFPPKAKSNQPK
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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