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Invitrogen™ Angiopoietin 4 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHANGPT4

362.68 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.00400kg

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Product Code. 15494893

  • £544.00 / 1 set
Estimated Shipment: 23-04-2024
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Description

Description

The Human Angiopoietin 4 (Hu APO4) ELISA quantitates Hu APO4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APO4. Principle of the method The Human APO4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. Ang-1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), is a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression is restricted to sites of vascular remodeling. Ang-3 and Ang-4 represent the mouse and human counterparts of the same gene locus. The structural divergence of Ang-3 and Ang-4 cause their divergent functions. Ang-3 and Ang-4 have very different distributions in their respective species, and Ang-3 appears to act as an antagonist while Ang-4 appears to function as an agonist. Ang-3 and Ang-4 share all the main structural characteristics of Ang-1 and Ang-2 and are homologous throughout the signal peptide, N-terminal region, coiled-coil segment and Fibrinogen-like domain.
Specifications

Specifications

Q9Y264
0.25 ng/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
51378
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Angiogenesis
2°C to 8°C
Human
4 hr. 45 min.
0.274-200 ng/mL
0.25ng/mL to 200ng/mL
Biotin
Proteases, Peptidases & Inhibitors
This ELISA antibody pair detects human Angiopoietin-4. Other species not determined.
Human
Colorimetric Microplate Reader
ANG3,ANG4
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
AGP4, ANG-3, ANG4
1 hr. 20 min.
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