Invitrogen™ Human alpha-2b Adrenergic Receptor (aa 198-247) Control Fragment Recombinant Protein

Product Code. 30198058

Brand: Invitrogen™ RP103896

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (57%), Rat (57%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-64258 (PA5-64258. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences.

Specifications

• Accession Number: P18089
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 151
• Name: Human alpha-2 b Adrenergic Receptor (aa 198-247) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: [a]2 B; ADA2B; Adra2b; Adra-2 b; ADRA2B adrenergic, alpha-2 B-, receptor; ADRA2L1; ADRA2RL1; ADRARL1; Adrenergic alpha2B- receptor class III; adrenergic receptor, alpha 2 b; adrenergic, alpha-2 B-, receptor; Adrenergic, alpha2B-, receptor class III; adrenoceptor alpha 2 B; alpha-2 adrenergic receptor subtype C2; alpha-2-adrenergic receptor-like 1; alpha2B; alpha-2 B adrenergic receptor; Alpha-2 B adrenoceptor; alpha-2 B adrenoreceptor; ALPHA2BAR; alpha-2 BAR; alpha2-C2; FAME2; G-protein coupled receptor; subtype alpha2-C2
• Common Name: alpha-2 b Adrenergic Receptor
• Gene Symbol: ADRA2B
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: IAKRSNRRGPRAKGGPGQGESKQPRPDHGGALASAKLPALASVASAREVN
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining