Invitrogen™ Human HTRA1, Recombinant Protein

Product Code. 11509091

Brand: Invitrogen™ RP77538

Description

Recombinant Protein

Secreted human HtrA1 serine protease is supposed to form oligomers of identical protein chains as revealed by X-ray analysis for the bacterial HtrA protein DegP. Polypeptide chains of human HtrA1 consist of several domains: An N-terminal insulin-like growth factor domain is followed by a Kazal-type serine protease inhibitor domain,a linker region,a trypsin-like protease domain and a PDZ domain. The function of HtrA1 appears closely linked to signaling by proteins of the TGFβ family. During mouse embryo development HtrA1 is localized in specific areas where signaling by TGFß family proteins occurs. HtrA1 binds TGFß,BMP4,Gdf5 and activin. It inhibits signaling by these factors. For inhibition serine protease activity of HtrA1 is essential. HtrA1 is upregulated in osteoarthritic cartilage. Increased cartilage HtrA1 may possibly aggravate osteoarthritis by antagonizing TGFβ and thereby promoting terminal differentiation of articular chondrocytes. In later stages of tumor progression HtrA1 is downregulated. As a protease HtrA1 hydrolyzes type II procollagen alpha1 C-propeptide,decorin and biglycan. Proteolytic activity of HtrA1 is regulated by ligand binding to the PDZ domain. RP-77538 is expressed in insect cells with a C-terminal His-tag. RP-77538 may be used in inhibition assays and as a control with corresponding anti-HtrA1 antibodies. RP-77538 appears as a major protein of about 53 kDa in SDS-PAGE.

Secreted human HtrA1 serine protease is supposed to form oligomers of identical protein chains as revealed by X-ray analysis for the bacterial HtrA protein DegP. Polypeptide chains of human HtrA1 consist of several domains: An N-terminal insulin-like growth factor domain is followed by a Kazal-type serine protease inhibitor domain, a linker region, a trypsin-like protease domain and a PDZ domain. The function of HtrA1 appears closely linked to signaling by proteins of the TGFbeta family. During mouse embryo development HtrA1 is localized in specific areas where signaling by TGFbeta family proteins occurs. HtrA1 binds TGFbeta, BMP4, Gdf5 and activin. It inhibits signaling by these factors. For inhibition serine protease activity of HtrA1 is essential. HtrA1 is upregulated in osteoarthritic cartilage. Increased cartilage HtrA1 may possibly aggravate osteoarthritis by antagonizing TGFbeta and thereby promoting terminal differentiation of articular chondrocytes. In later stages of tumor progression HtrA1 is downregulated. As a protease HtrA1 hydrolyzes type II procollagen alpha1 C-propeptide, decorin and biglycan. Proteolytic activity of HtrA1 is regulated by ligand binding to the PDZ domain. RP-77538 is expressed in insect cells with a C-terminal His-tag. RP-77538 may be used in inhibition assays and as a control with corresponding anti-HtrA1 antibodies. RP-77538 appears as a major protein of about 53 kDa in SDS-PAGE.

Specifications

• Accession Number: Q92743
• Concentration: 0.2mg/mL
• For Use With (Application): Control, Inhibition Assay
• Formulation: 50mM tris HCl with 0.05% Brij-35,150mM NaCl,50mM Imidazole,5mM CaCl2 and no preservative; pH 7.5
• Gene ID (Entrez): 5654
• Name: Human HTRA1
• pH Range: 7.5
• Quantity: 5 μg
• Source: Insect cells
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Common Name: Human HTRA1
• Gene Symbol: HTRA1
• Specific Reactivity: Human
• Conjugate: Unconjugated
• Cross Reactivity: Hu
• Species: Baculovirus
• Recombinant: Recombinant
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: purified