Novus Biologicals™ HSP27 Antibody Pair [HRP]

Product Code. 18214198

Brand: Novus Biologicals™ NBP27957915PLATES

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human HSP27.

Specifications

• Assay Range: 9.4 pg/mL
• Assay Sensitivity: 9.38-600 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human HSP27.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: 28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
• Gene ID (Entrez): 3315
• Gene Symbol: HSPB1
• Host Species: Mouse/Rabbit
• Kit Contents:
  Detection Antibody: 0.5 mg/mL mouse anti-HSP27 monoclonal antibody conjugated to horseradish-peroxidase (HRP).
  Capture Antibody: 1.0 mg/mL of rabbit anti-HSP27 monoclonal antibody,
  Standard: Each vial contains 13 ng of recombinant HSP27.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience, Phospho Specific
• Storage Requirements: Storage is content dependent.
• Target: HSP27
• Technique: 1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.6 ng/mL is recommended.