Invitrogen™ HP1 alpha Recombinant Human Monoclonal Antibody (RAB-C133)

Human Recombinant Monoclonal Antibody

Brand:  Invitrogen™ MA551856

Product Code. 30087895

Description

This antibody recognizes CBX5 (Chromobox protein homolog 5). It binds to a folded domain, amino acids 18-75. CBX5 is a component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression.

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

Specifications

• Antigen: HP1 alpha
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Gene Accession No.: P45973
• Gene Symbols: Cbx5
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing CBX5 protein under non-denaturing conditions.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: F(ab) κ

• Applications: ChIP Assay, ELISA, Mass Spectrometry
• Clone: RAB-C133
• Conjugate: Unconjugated
• Gene: Cbx5
• Gene Alias: 2610029O15Rik; antigen p25; C75991; Cbx5; Cbx5 antibody; chromobox 5; chromobox homolog 5; chromobox homolog 5 (HP1 alpha homolog, Drosophila); chromobox protein homolog 5; chromobox-like protein 5; epididymis luminal protein 25; HEL25; heterochromatin protein 1 alpha; heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP1; HP1 alpha; HP-1 alpha antibody; HP1 alpha homolog; Hp1a; Hp1alpha; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha; mHP1(alpha)
• Host Species: Human
• Purification Method: Metal-chelate chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23468
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid