HLA-G, PE, clone: 87G, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9957-42

Product Code. 15587286

Description

Description: The monoclonal antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses. HLA-G has seven reported isoforms. The antibody 87G recognizes both HLA-G1 and the solube HLA-G5. Expression of HLA-G is found primarily in fetal trophoblast cells as they invade the maternal decidua thereby protecting the fetus from the maternal immune system. Like the highly mitotic trophoblast, abundant HLA-G protein expression has been identified in some tumors, including melanoma, breast carcinoma and renal carcinoma as well as CLL, AML and B-CLL. Some expression has also been found in pancreatic islets, erythroid and endothelial progenitors and the adult thymic medulla.HLA-G+ CD4 or CD8 cells have been identified in normal human peripheral blood and are thought to act as regulatory cells in that they are hypoproliferative with a unique cytokine profile differing from Tregs. The receptors for HLA-G are CD85j/ILT2, CD85d/ILT4, and CD158. Recent studies have shown a role for HLA-G in tolerance and maintenance of transplanted organs.Applications Reported: This 87G antibody has been reported for use in flow cytometric analysis.Applications Tested: This 87G antibody has been pre-titrated and tested by flow cytometric analysis of stimulated U937 cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: HLA-G
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.2% BSA, 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P17693
• Gene Alias: DADB-15K14.8, MHC-G
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow Cytometry
• Clone: 87G
• Conjugate: PE
• Gene: HLA-G
• Format: Conjugated
• Gene Symbols: HLA-G
• Isotype: IgG2a, kappa
• Quantity: 100 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 3135

For Research Use Only.