HLA-E, Functional Grade, clone: 3D12HLA-E, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-9953-85

Product Code. 15567836

Description

Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.Applications Reported: This 3D12HLA-E antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12HLA-E antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.

Specifications

• Antigen: HLA-E
• Classification: Monoclonal
• Concentration: 1mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P13747
• Gene Alias: DAQB-90C11.16, FLJ26655, HLAA
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow CytometryþFunctional Assay
• Clone: 3D12HLA-E
• Conjugate: Functional Grade
• Gene: HLA-E
• Format: Purified
• Gene Symbols: HLA-E
• Isotype: IgG1, kappa
• Quantity: 500μg
• Storage Requirements: Store at 2-8°C.
• Primary or Secondary: Primary
• Gene ID (Entrez): 3133

For Research Use Only.