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HLA-E, Functional Grade, clone: 3D12HLA-E, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-9953-85
Code : NEW
Additional Details : Weight : 0.09500kg
Description
Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.Applications Reported: This 3D12HLA-E antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12HLA-E antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Specifications
HLA-E | |
Monoclonal | |
1mg/mL | |
PBS with no preservative; pH 7.2 | |
P13747 | |
DAQB-90C11.16, FLJ26655, HLAA | |
Murine | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Human |
Functional Assay | |
3D12HLA-E | |
Functional Grade | |
HLA-E | |
Purified | |
HLA-E | |
IgG1, kappa | |
500μg | |
Store at 2-8°C. | |
Primary | |
3133 |
For Research Use Only.