Invitrogen™ HLA-DRB4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA587621

Product Code. 16536071

Description

Immunogen sequence: GDTQPRFLEQ AKCECHFLNG TERVWNLIRY IYNQEEYARY NSDLGEYQAV TELGRPDAEY WNSQKDLLER RRAEVDTYCR YNYGVVESFT VQRRVQPKVT VYPSKTQPLQ HHNLLVCSVN GFYPGSIEVR WFRNGQEEKA GVVSTGLIQN GDWTFQTLVM LETVPRSGEV YTCQVEHPSM MSPLTVQWSA RSESAQSK; Positive Samples: Raji, Mouse brain; Cellular Location: Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.

HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-DRB4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HLA-DRB4
• Gene Alias: DR4; DR-4; DRB1 transplantation antigen; DRB4; HLA class II histocompatibility antigen, DR beta 4 chain; HLA-DR4B; HLA-DRB4; human leucocyte antigen DRB4; leukocyte antigen; major histocompatibility complex, class II, DR beta 4; MHC class II antigen DRB4; MHC class II antigen HLA-DR-beta; MHC class2 antigen; MHC HLA DR-beta chain
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3126
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.49 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P13762
• Gene Symbols: HLA-DRB4
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-227 of human HLA-DRB4 (NP_0688184).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG