Invitrogen™ HLA-DMA Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA522365

Product Code. 13239668

Description

Recommended positive controls: HeLa, HepG2, Jurkat, Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-DMA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HLA-DMA
• Gene Alias: class II histocompatibility antigen, M alpha chain; D6S222E; DAAP-27A1.5; DMA; HLA class II histocompatibility antigen, DM alpha chain; HLADM; HLA-DMA; major histocompatibility complex, class II, DM alpha; MHC class II antigen DMA; really interesting new gene 6 protein; RING6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3108
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P28067
• Gene Symbols: HLA-DMA
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HLA-DMA. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG