HLA-BC Monoclonal Antibody (B1.23.2), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 17-5935-41

Product Code. 15508166

Description

Description: This B1.23.2 monoclonal antibody recognizes the human Human Leukocyte Antigens (HLA) B and C. Along with HLA-A, these proteins correspond to MHC Class I in humans. HLA-B and HLA-C are expressed on nearly all cell types and heterodimerize with beta-2 microglobulin at the cell surface. These molecular complexes play a critical role in the presentation of endoplasmic reticulum-derived peptides to immune cells. Applications Reported: This B1.23.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This B1.23.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. maligt cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Specifications

• Antigen: HLA-BC
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01889
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Flow Cytometry
• Clone: B1.23.2
• Conjugate: APC
• Gene: HLA-B
• Gene Symbols: HLA-B
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3106
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

For Research Use Only.