HIV1 Tat Monoclonal Antibody (N3), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA171511

Product Code. 11530352

Description

This antibody reacts with HIV-1 Tat strains HXB2, BH10, IIIB and HAN. It has been mapped to amino acids 47-58 (YGKKRRQRRRA – B subtype a.a. consensus, Tat basic domain). MA1-71511 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat. This clone can also be referred to as N3 (02-010).

HIV-1 Tat ("Trans-Activator of Transcription") consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.

Specifications

• Antigen: HIV1 Tat
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Gene Alias: Human immunodeficiency virus; HIV-1 Tat
• Immunogen: Recombit Tat protein of HIV-1 HXB2 purified from E. coli
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ChIP Assay, ELISA, Western Blot
• Clone: N3
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: IgM
• Regulatory Status: RUO
• Target Species: Virus
• Product Type: Antibody
• Isotype: IgM