Histone H3, Trimethyl Lys9 Antibody (6F12-H4), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 19 publications

Product Code	Brand	Quantity
18715383	Novus Biologicals NBP1-30141SS	0.025 mL
18731484	Novus Biologicals NBP1-30141	0.1 mL

Description

Histone H3 Monoclonal specifically detects Histone H3 in Human, Mouse, Rat, Porcine, C. elegans, Drosophila, Invertebrate, Mammal, Yeast samples. It is validated for Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Histone H3 (Trimethyl Lys9)
• Concentration: 1 mg/mL
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Chromatin Modifiers, Chromatin Research, Epigenetics, Histones and Modified Histones, Loading Controls
• Gene Accession No.: P84243, P84244
• Gene ID (Entrez): 126961
• Immunogen: This Histone H3 [Trimethyl Lys9] antibody (6F12-H4) was raised against a synthetic peptide made to an N-terminal region of Histone H3 (between amino acids 1-50). [UniProt# P84243]
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 15 kDa

• Clone: 6F12-H4
• Dilution: Western Blot 1:2000, ELISA 1:100-1:2000, Immunohistochemistry 1:1000. Use reported in scientific literature (PMID 23872316), Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 28270554), Immunohistochemistry-Paraffin 1:1000, Chromatin Immunoprecipitation (ChIP) 1:10-1:500
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, C. elegans, Drosophila, Invertebrate, Mammalia, Yeast
• Gene Alias: H3, H3 histone, family 3A, H3.3A, H3.3B, H3F2, H3F3, H3F3B, H3FM, H3K9Me3, HIST2H3C, histone H3.3, MGC87782, MGC87783
• Gene Symbols: H3C14
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: All mammals including human, mouse and rat, Drosophila, C. elegans, yeast. Invertebrate/Blattella germanica (German cockroach) reactivity reported in scientific literature (PMID: 23872316).

For Research Use Only