Thermo Scientific™ HisProbe™-HRP Reagent and Kit

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

Brand: Thermo Scientific™

Manufacturer Part Number: 15165

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 1.81440kg

Product Code. 10208163

Quantity Price
1 £ 195.0 / 2mg
EU Stock 8
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Description and Specification


Storage Requirements Store HisProbe-HRP, Blocker BSA in TBS (10X), and Surfact-Amps 20 at 4°C. All other components may be stored at room temperature.
Formulation Lyophilized powder from protein in MES buffer, pH 5.5
Kit Contents Single component
Conjugate HRP
Sufficient For Preparing approx. 2L of 1X probing solution
Description Pierce HisProbe-HRP Conjugate
Quantity 2mg

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).


  • Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol

  • Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies

  • High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags

  • Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies