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His-Tag Mouse anti-Tag, Clone: 1B7G5, Proteintech

Product Code. 16857495 Shop All Proteintech Products
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150 μL
Unit Size:
150µL
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16857495 150 μL 150µL
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Product Code. 16857495 Supplier Proteintech Supplier No. 660051IG150UL

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Description

Mouse Monoclonal Antibody

The His-tag antibody is a useful tool for monitoring His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering. By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.

Specifications

Antigen His-Tag
Applications Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Classification Monoclonal
Clone 1B7G5
Concentration 1.34 mg/mL
Conjugate Unconjugated
Formulation PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
Gene Alias 6x His, 6x His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED
Host Species Mouse
Immunogen Peptide
Purification Method Protein G
Quantity 150 μL
Regulatory Status RUO
Primary or Secondary Primary
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Tag
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