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Cytiva His SpinTrap™ Spin Columns and Kit

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns.

Brand:  Cytiva 28-9321-71

Code : GE

Additional Details : Weight : 0.43200kg Transport : UN number : 3267 Chem class : 8 Pack group : III


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Product Code. 10157094

  • £330.01 / 1 L

In stock, ready to ship: 2
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Description

Description

  • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
  • One run in standard microcentrifuge takes approximatedly 10 min.
  • Yields up to 750μg purified protein per column
  • Allows direct purification of both clarified and unclarifed cell lysates
  • Also useful for screening large numbers of small-scale bacterial lysates
  • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Stable (long-term) in the pH range 3-12
  • Average particle size: 34μm

Specifications

Specifications

1 Piece
His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)
2 to 14
His SpinTrap Kit
1 Pack His SpinTrap and 1 Pack His Buffer Kit
6% Highly Cross-linked Agarose
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Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Skin sensitiser Category 1
  • Skin corrosion/irritation Category 1 C
  • Reproductive toxicity Category 1B

Hazard Statement
  • H317-May cause an allergic skin reaction.
  • H314-Causes severe skin burns and eye damage.
  • H360D-May damage the unborn child.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P331-Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P305-IF IN EYES:Immediately call a POISON CENTER/doctor..
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Nickel, imidazole
  • UNKNOWN AQUATIC-Contains 15% of components with unknown hazards to the aquatic environment.

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