Cytiva His SpinTrap™ Spin Columns

Product Code.	Type	unitSize
10157094	Spin Column Kit	1 L
11733319	Spin Column	Pack of 50

Product Code. 10157094 Supplier Cytiva Supplier No. 28932171

Description

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

 

• Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
• One run in standard microcentrifuge takes approximatedly 10 min.
• Yields up to 750μg purified protein per column
• Allows direct purification of both clarified and unclarifed cell lysates
• Also useful for screening large numbers of small-scale bacterial lysates
• Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
• Kit components (spin columns and buffer) can also be purchased separately

 

Medium Characteristics:

• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• Stable (long-term) in the pH range 3-12
• Average particle size: 34μm

 

Order Info

Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)

Specifications

• Kit Contents: 1 Pk. His SpinTrap (28-4013-53) and 1 Pk. His Buffer Kit (11-0034-00)
• pH Range: 2 to 14
• Support Type: 6% Highly Cross-linked Agarose
• Content And Storage: 4°C to 30°C
• Quantity: 1 Piece
• Type: Spin Column Kit

Safety and Handling

Signal Word

• Danger||

Hazard Category

• Skin sensitiser Category 1
• Skin corrosion/irritation Category 1 C
• Reproductive toxicity Category 1B

Hazard Statement

• H317-May cause an allergic skin reaction.
• H314-Causes severe skin burns and eye damage.
• H360D-May damage the unborn child.

Precautionary Statement

• P201-Obtain special instructions before use.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P331-Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P310-Immediately call a POISON CENTER/doctor/.
• P305-IF IN EYES:Immediately call a POISON CENTER/doctor..
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contain: Nickel, imidazole
• UNKNOWN AQUATIC-Contains 15% of components with unknown hazards to the aquatic environment.