HIF1A 293T Cell Overexpression Lysate (Denatured), Abnova

Product Code.	Quantity	unitSize
16126111	100 μL	100µL

Product Code. 16126111 Supplier Abnova Supplier No. H00003091T01.100uL

Description

Denatured HIF1A 293T Cell Overexpression Lysate

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq)

• Transfected cell line: 293T
• Plasmid: pCMV-HIF1A full-length
• Host: Human
• Molecular weight: 80.96kDa
• Storage Buffer: 1X Sample Buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Western Blotting

Specifications

• Cell Line: 293T
• For Use With (Application): Western Blot
• Description: HIF1A 293T Cell Transient Overexpression Lysate(Denatured)
• Gene Accession No.: NM_181054
• Molecular Weight (g/mol): 82.7
• pH Range: 6.8
• Plasmid: pCMV-HIF1A full-length
• Quality Control Testing: Transient overexpression cell Lysate was tested with Anti-HIF1A antibody (H00003091-B01) by western blotting
• Quantity: 100 μL
• Storage Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
• Host Species: Human
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Safety and Handling

Product Identifier

• 100UL HIF1A 293T Cell Transient Overexpression Lys

Signal Word

• Warning

Hazard Category

• Skin sensitiser Category 1

Hazard Statement

• H317-May cause an allergic skin reaction.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.

Supplemental information

• MIXTURE LIST-Contain: Mercaptoethanol