HIF-1 alpha Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 241 publication

Product Code	Brand	Quantity
18522480	Novus Biologicals NB100-449SS	0.025 mL

Description

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Canine, Chicken, Goat, Primate, Monkey samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Knockout Validated, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Dilution: Western Blot 1:2000 - 1:10000, Simple Western 1:200, Flow Cytometry 0.125 μg per 1 million cells in a 150 mcl reaction, ELISA 1:100-1:2000, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 2-5 μg/mg lysate, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Knockout Validated, Knockdown Validated
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Formulation: TBS, 0.1% BSA with 0.09% Sodium Azide
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Gene Symbols: HIF1A
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: Monkey (COS-7) and Rat (review feedback). Has been shown to detect HIF-1 alpha in both mouse and human tissue by IHC with Citrate buffer antigen retrieval. Based on 100% sequence identity, this antibody is expected to react with: Cavia porcellus, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Grass carp, Northern pike, Atlantic cod, Duckbill platypus and Gansu zokor.

• Concentration: 0.2 mg/mL
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: Q16665
• Gene ID (Entrez): 3091
• Immunogen: The immunogen recognized by this HIF-1 alpha Antibody maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1 [Uniprot# Q16665].
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C. Do not freeze.
• Molecular Weight of Antigen: 93 kDa

For Research Use Only