MP Biomedicals HCV Blot 3.0 (RUO)

Product Code. 30694941

Brand: MP Biomedicals 11132036

Description

The Blot is a qualitative enzyme immunoassay for the in vitro detection of antibodies to hepatitis C virus (HCV) in human serum or plasma.

• The nitrocellulose strips are coated with four recombinant HCV proteins from the Capsid, NS3, NS4, and NS5 regions of the HCV genome.
• The HCV proteins are expressed as GST fusion proteins, so a GST control band is included to indicate reactivity to native GST.
• The blots also contain an IgG control band and an anti-IgG band.
• Individual nitrocellulose strips are incubated with diluted serum or plasma specimens and controls.
• Specific antibodies to HCV, if present in the specimen, will bind to the HCV proteins on the strips.
• The strips are washed to remove unbound materials and then incubated with affinity purified anti-human IgG conjugated with alkaline phosphatase.
• The conjugate antibody will bind to any antigen-antibody complexes formed on the blots.
• Unbound conjugate is removed by washing. A BCIP/NBT substrate is added to visualise reactive protein bands on the blots.
• The HCV BLOT 3.0 includes structural and non-structural antigens from HCV and is intended as supplemental confirmatory assay for presence of antibodies to HCV.
• It is intended for use as a more specific supplemental test on specimens found repeatedly reactive using specific procedures such as ELISA.
• Research Use Only (RUO).
• For professional use only.
• Human serum and plasma.

Specifications

• Product Type: Western Blot Kit
• Detection Method: Colorimetric
• For Use With (Application): Immunoblot (Western)
• Membrane Compatibility: Nitrocellulose
• Quantity: 36 Tests/Kit
• Substrate: Alkaline Phosphatase