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Gibco™ Ham's F-10 Nutrient Mix
Description
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ F-10 product made in our Grand Island facility. This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ F-10 product made in our Grand Island facility. This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications
Specifications
| Cell Line | CHO |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | Ham's F-10 Nutrient Mix |
| With Additives | Glutamine, Phenol Red, Sodium Pyruvate |
| Without Additives | No HEPES |
| For Use With (Application) | Mammalian cell culture |
| Quantity | 500 mL |
| Certifications/Compliance | ISO 13485 standards, FDA |
| pH | 7.0 to 7.4 |
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Product Title
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