Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

Product Code.	Quantity	unitSize
11824111	10 mL	10mL
10444414	24 x 100 μL	Each
11804111	5 mL	5mL
10320015	1 mL	1mL

Product Code. 11824111 Supplier Thermo Scientific™ Supplier No. 78439

Description

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific Halt Protease Inhibitor Cocktail, EDTA-free (100X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction.

Features of Halt Protease Inhibitor Cocktail, EDTA-free (100X):

• Convenient—the refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need
• No proprietary ingredients—fully disclosed formulation contains optimized concentrations of six broad-spectrum protease inhibitors AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A stabilized in high-quality dimethylsulfoxide (DMSO)
• Multiple package sizes—choose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination
• EDTA-free formulations—ensures compatibility with isoelectric focusing
• Lysis buffer compatible—use with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent. Add 10 μL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract.

Applications:

• Protection of intact, active cellular proteins from degradation by endogenous proteases
• Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Pierce Cell Lysis Reagents, other commercially formulated cell or tissue lysis products, sonication, French press, blender homogenization or repetitive freeze/thaw cycling
• Screening of extracts for proteolytic activity
• The study of proteolysis in the regulation of cellular processes

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types. Proteases are regulated and compartmentalized in living cells, but cell lysis releases and mixes them with other extracted proteins. Their activity can thus lead to the loss of a large number of valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases. Halt Protease Inhibitor Cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively.

Related Products

• Halt™ Protease Inhibitor Single-Use Cocktail (100X) (Cat. No. 78430)

Specifications

• Product Type: Protease Inhibitor Cocktail
• Content And Storage: Upon receipt store at 4°C
• Form: Liquid
• Product Line: Halt
• Buffer: Lysis Buffer
• Reagent Type: Protease Inhibitor
• Inhibitors: Protease Inhibitor
• Quantity: 10 mL

Safety and Handling

Product Identifier

• Protease Inhibitor Cocktail, EDTA-Free (100X)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contain: Benzenesulfonyl fluoride, 4- (2-aminoethyl)-, hydrochloride

For Research Use Only. Not for use in diagnostic procedures.