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Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.

£192.00 - £762.00

Specifications

Product Type Phosphatase Inhibitor Cocktail
Content And Storage Upon receipt store product at 4°C. Do not freeze.
Form Liquid
Product Line Halt™
Reagent Type Phosphatase Inhibitor
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Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10668304
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Thermo Scientific™
78420
1 mL
£192.00
1mL
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11833955
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Thermo Scientific™
78428
24 x 100 μL
£298.00
Each
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11814101
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Thermo Scientific™
78426
5 x 1 mL
£562.00
5mL
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11824101
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Thermo Scientific™
78427
10 mL
£762.00
10mL
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Estimated Shipment: 10-04-2024
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Description

Description

Preserve phosphorylation of proteins in cells and tissues with Thermo Scientific™ Halt Phosphatase Inhibitor Cocktail.

  • Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases, as well as protein tyrosine phosphatases (PTPs) during cell lysis
  • The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate
  • Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract
  • The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays
  • Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell including signal transduction, cell division, and apoptosis
  • To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis

Additional Features

  • Devoid of protease inhibitors – To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail (78420)
  • Dialyzable – Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components
  • Supplied as a 100X concentrate – effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X
  • Compatibility – compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions
  • All-in-one format – inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
  • Compatible with popular protein assays – works with standard protein assays, including BCA and coomassie (Bradford) assays
  • Convenient storage – refrigerator storage (2 to 8°C) saves valuable space in the freezer and allows for immediate use
Specifications

Specifications

Phosphatase Inhibitor Cocktail
Liquid
Phosphatase Inhibitor
Upon receipt store product at 4°C. Do not freeze.
Halt™
Phosphatase Inhibitor
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.