Invitrogen™ H4K20me3 Monoclonal Antibody (6F8-D9)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA518074

Product Code. 15384341

Description

This antibody shows weak cross-reactivity with K20 di-methylated histone H4, but does not react with histone H3 or mono- or unmethylated K20 histone 4. This antibody is predicted to react with Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), and mammals. This target has a predicted molecular weight of 11 kDa.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Specifications

• Antigen: H4K20me3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: P62805, Q27894
• Gene Symbols: H4-16, H4C1, H4C11, H4C14, H4C2, H4C5, H4C9, his-4
• Immunogen: Synthetic peptide of Human Histone H4 (Immunogen sequence: 15-AKRHR(Kme3)VLRDN-25 (branched)).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, C. elegans
• Product Type: Antibody
• Isotype: IgG1

• Applications: ChIP Assay, Dot Blot, ELISA, Western Blot
• Clone: 6F8-D9
• Conjugate: Unconjugated
• Gene: H4C1
• Gene Alias: CELE_T10C6.11; F07B7.11; F07B7.4; F45F2.12; FO108; H4; H4 histone family, member A; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K20; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/j; H4/K; H4/M; H4/n; H4/O; H4/p; H4-12; H4-16; H4-53; H4C1; H4c11; H4C12; H4C13; H4c14; H4C15; H4C2; H4C3; H4C4; H4C5; H4c6; H4C8; H4C9; H4f16; H4F2; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4K20; H4Lys20me3; H4M; his-20; his-22; his-4; his-52; his-54; his-8; HIST1H4; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; Hist1h4h; Hist1h4i; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; Hist2h4; HIST2H4A; HIST2H4B; Hist4h4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; histone cluster 1, H4a; histone cluster 1, H4b; histone cluster 1, H4e; histone cluster 1, H4i; histone cluster 2, H4; histone cluster 2, H4a; Histone Cluster 4; histone cluster 4, H4; histone family member; Histone H2B 2; Histone H4; Histone H4A; Histone H4K20me3; histone IV, family 2; K06C4.12; K06C4.4; methyl Histone 3; methyl Histone H3; T10C6.11; Trimethyl H4; Tri-methyl H4; Trimethyl Histone H4; Tri-methyl Histone H4; Tri-Methyl-H4 K20; Tri-Methyl-H4 Lys20; Tri-Methyl-Histone H4 K20; Tri-Methyl-Histone H4 Lys20; X04652
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 121504, 180072, 8294, 8359, 8363, 8366, 8367, 8370
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid