Thermo Scientific Chemicals Guanidine thiocyanate, Molecular Biology Grade

Product Code. 15486719

Brand: Thermo Scientific Chemicals J65104.18

Description

A protein denaturant | CAS: 593-84-0 | CH₅N₃·CHNS

Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.

Solubility
Soluble in water (1420 g/L) at 20°C, and ethanol (5%).

Notes
Light Sensitive. Keep container tightly closed. Store away from oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 593-84-0
• Molecular Formula: CH₅N₃·CHNS
• MDL Number: MFCD00013027
• Synonym: Guanidine rhodanide

• Melting Point: 118°C to 122°C
• Color: White
• Density: 1.29
• Quantity: 50 g
• Beilstein: 3563461
• Sensitivity: Light sensitive
• Solubility Information: Soluble in water (1420g/L) at 20°C,and ethanol (5%).
• Formula Weight: 118.16
• Grade: Molecular Biology
• Physical Form: Powder
• Chemical Name or Material: Guanidine thiocyanate

Safety and Handling

Product Identifier

• Guanidine thiocyanate, Molecular Biology Grade

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 1 C

Hazard Statement

• H302-Harmful if swallowed.
• H312-Harmful in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H318-Causes serious eye damage.
• H332-Harmful if inhaled.
• H412-Harmful to aquatic life with long lasting effects.
• EUH032-Contact with acids liberates very toxic gas.
• EUH071-Corrosive to the respiratory tract.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.

Supplemental information

• MIXTURE LIST-Contains : Thiocyanic acid

SDS

RUO – Research Use Only