Guanidine Hydrochloride, Ultra Pure grade, MP Biomedicals

Description

• Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins
• This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies
• This can be used as the first step in refolding proteins or enzymes into their active form
• Urea and dithiothreitol (DTT) may also be necessary
• Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties
• It is an inhibitor of RNase
• Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins
• It apparently disrupts hydrogen bonds which hold the protein in its unique structure; however, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions

Specifications

• Melting Point: 180°C to 190°C
• Color: White
• Linear Formula: CH5N3·HCl
• Quantity: 100 g
• Solubility Information: Soluble in water (6 M - clear, colorless solution).
• Formula Weight: 95.53
• Percent Purity: ≥99%
• Grade: Ultra Pure
• Physical Form: Crystalline Powder
• Chemical Name or Material: GUANIDINE HYDROCHLORIDE

Safety and Handling

Product Identifier

• Guanidine Hydrochloride, ultra pure

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P321-Specific treatment (see on this label).
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : guanadine hydrochloride