GRP78 Monoclonal Antibody (1H11-1H7), APC, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA545276

Product Code. 17837372

Description

0.5 μg/mL of MA5-45276 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis.∣Detects approximately 78kDa.

GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78’s involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.

Specifications

• Antigen: GRP78
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.15mM MES, 47.35mM phosphate with 1.033mM EDTA and no preservative; pH 7.4
• Gene Accession No.: P06761, P11021, P20029, Q0VCX2, Q91883
• Gene Symbols: Hspa5
• Immunogen: His-tagged human GRP78
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Bovine, Fungi, Hamster, Human, Mouse, Non-human Primate, Rabbit, Rat, X. laevis
• Form: Liquid

• Applications: ELISA, Immunocytochemistry, Western Blot
• Clone: 1H11-1H7
• Conjugate: APC
• Gene: Hspa5
• Gene Alias: 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein homolog; 78-kD glucose-regulated protein precursor; AL022860; AU019543; baffled; Binding-immunoglobulin protein; bip; BiP protein; BiP/Grp78; cb865; D2Wsu141e; D2Wsu17e; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; fb60h09; fi36d04; FLJ26106; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein, 78kDa; GRP 78; grp78; GRP-78; heat shock 70 kDa protein 5; heat shock 70 kDa protein 5a; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5; heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; heat shock protein 70 family protein 5; heat shock protein family A (Hsp70) membe
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100008764, 100689305, 14828, 25617, 3309, 397850, 415113
• Content And Storage: 4° C
• Isotype: IgG2b