GRP78/HSPA5 Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 25 publications

Product Code	Brand	Quantity
18019123	Novus Biologicals NBP1-06274SS	0.025 mL
18708453	Novus Biologicals NBP1-06274	0.1 mL

Description

GRP78/HSPA5 Polyclonal specifically detects GRP78/HSPA5 in Human, Mouse, Rat, C. elegans, Chicken, Drosophila, Sheep, Zebrafish samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry - Free Floating.

Specifications

• Antigen: GRP78/HSPA5
• Dilution: Western Blot 0.5 ug/ml, Simple Western 1:25, Flow Cytometry 1:150. Use reported in scientific literature (PMID 20208072), Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:50. Use reported in scientific literature (PMID 24089213), Immunohistochemistry-Paraffin reported in scientific literature (PMID 24089213), Immunohistochemistry Free-Floating reported in scientific literature (PMID 26329458)
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Cancer, Cellular Markers, ER Markers, Lipid and Metabolism, Unfolded Protein Response, Unfolded Protein Response (UPR)
• Formulation: PBS with 0.02% Sodium Azide
• Gene Alias: 78 kDa glucose-regulated protein, BiP, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, FLJ26106, GRP-78, GRP78BIP, Heat shock 70 kDa protein 5, heat shock 70kD protein 5 (glucose-regulated protein, 78kD), heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), Immunoglobulin heavy chain-binding protein, MIF2
• Gene Symbols: HSPA5
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: Human, mouse, rat, sheep and chicken. Predicted to react with Zebrafish, S. cerevisiae, primate, bovine, and Drosophila based on 100% sequence homology.

• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Free Floating)
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, C. elegans, Chicken, Drosophila, Sheep
• Gene Accession No.: P11021
• Gene ID (Entrez): 3309
• Immunogen: Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 78 kDa

For Research Use Only