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Thermo Scientific™ pTK-Green Renilla Luc Vector for Luciferase Assays

Product Code. 12182230 Shop All Thermo Scientific Products
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12182230 10 μg 10µg
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Product Code. 12182230 Supplier Thermo Scientific™ Supplier No. 16154
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    £94.71 / 10µg
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Estimated Shipment: 20-11-2026
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Description

Drive Green Renilla luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pTK-Green Renilla Luc vector:

  • Weak (TK) constitutive promoter for co-transfection and normalization
  • Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin

Related Products

pMCS-Green Renilla Luc Vector for Luciferase Assays (Cat. No. 16152)

pCMV-Green Renilla Luc Vector for Luciferase Assays (Cat. No. 16153)

TRUSTED_SUSTAINABILITY

Specifications

Description pTK-Green Renilla Luc Vector
Type Luc Vector
Cloning Method Restriction Enzyme/MCS
Delivery Type Transfection
Quantity 10 μg
Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Reporter Gene Green Renilla Luciferase
Selection Agent (Eukaryotic) Puromycin
Product Line Pierce
For Use With (Application) Reporter Assays
Promoter TK
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For Research Use Only. Not for use in diagnostic procedures.

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