Granzyme B Rat anti-Mouse, Biotin, Clone: 16G6, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 13-8822-82

100UG ANTI-MOUSE GRANZYME B BIOTIN

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15390070

Quantity Price
1 £ 201.0 / 100µg
Estimated Shipment date
from Supplier 21-02-2017
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Description and Specification

Specification

Antigen Granzyme B
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Rat
Applications Flow Cytometry (Intracellular Staining)
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Western Blotting
Isotype IgG2b
Quantity 100μg
Regulatory Status RUO
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Clone 16G6
Gene Alias GrzB, GrB
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze.
Concentration 0.5mg/mL
Conjugate Biotin

The 16G6 antibody reacts with mouse Granzyme B (GrB) which is a member of the granzyme serine protease family. GrB is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis.

For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (cat. 00-5523). Other buffers may yield varying results. For more information, please contact technical support at tech@ebioscience.com.