missing translation for 'onlineSavingsMsg'
Learn More

GPNMB, eFluor 660, clone: CTSREVL, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5708-80

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15579976

  • £68.96 / 25µg


EU Stock 1
Add to basket

Description

Description

Description: This CTSREVL monoclonal antibody recognizes human GPNMB also known as Osteoactivin, DC-HIL and HGFIN. This type-1 transmembrane glycoprotein was described independently as a bone development factor, tumor related molecule, neurokinin, and immune regulator. Expressed on macrophages and dendritic cells, GPNMB is a ligand for proteoglycan Syndecan-4 (SD-4), which is found on T cells and is known to inhibit their activation and proliferation. Hence, surface expression of GPNMB by myeloid cells may be indicative of their tolerogenic properties. Various cancers express GPNMB, and it is suspected to help in immune evasion. Treatment with IL-10 and various immunosuppressive drugs (e.g., dexamethasone) upregulate GPNMB on macrophages and dendritic cells. GPNMB may also be secreted.The CTSREVL antibody will work on paraformaldehyde-fixed cells.Applications Reported: This CTSREVL antibody has been reported for use in flow cytometric analysis.Applications Tested: This CTSREVL antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

gpnmb
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q99P91
HGFIN, NMB, glycoprotein NMB, glycoprotein nmb-like protein, osteoactivin, transmembrane glycoprotein HGFIN, transmembrane glycoprotein NMB
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
CTSREVL
eFluor 660
Gpnmb
Conjugated
Gpnmb
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
93695
Videos

For Research Use Only.