Invitrogen™ Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, PE

Goat Polyclonal Secondary Antibody

Brand:  Invitrogen™ 31863

Product Code. 10056673

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31863 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31863 reacts with the heavy chains of mouse IgG2a, but not with heavy chains of other mouse IgG subclasses or with light chains of most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins. However, these antibodies may cross-react with immunoglobulins from other species. Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 82.0 at 566 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) Phycoerythrin Source: Seaweed Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. RPE Amax= 490 nm, 545 nm, and 566 nm; Emax= 580 nm. Reconstitute with 0.5 mL of distilled water (0.5 mg/mL after restoration). Country of Origin: USA.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG2a Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: PE
• Host Species: Goat
• Quantity: 500 μL
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Lyophilized

• Applications: Flow Cytometry
• Concentration: 0.5 mg/mL
• Formulation: PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Secondary Antibody
• Isotype: IgG