Invitrogen™ Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488

Product Code.	Quantity	unitSize
10696113	1 mg	500µL

Product Code. 10696113 Supplier Invitrogen™ Supplier No. A11029

Description

Goat Polyclonal Secondary Antibody

To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been highly cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in 'highly cross-adsorbed' preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Flu...

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG (H+L) Highly Cross-Adsorbed
• Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 2 mg/mL
• Conjugate: Alexa Fluor 488
• Formulation: PBS with 5mM sodium azide; pH 7.5
• Host Species: Goat
• Immunogen: Gamma Immunoglobins Heavy and Light chains.
• Purification Method: Purified
• Quantity: 1 mg
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Mouse
• Content And Storage: 4°C, store in dark
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

What is the difference between Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Cat. No. A11001) and Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Cat. No. A11029)?

The difference between the highly cross adsorbed and cross adsorbed antibodies is the amount of different IgG of different species the antibodies have been allowed to react with during the purification phase in order to reduce the chance of cross reactivity of the antibody. In this case the cross adsorbed has been reacted with the human IgG and human serum prior to conjugation, whereas the highly cross adsorbed against the bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum. This results in less non‐specific staining.