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Invitrogen™ Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Description
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum immunoglobulins, human IgG or serum proteins. Based on IEP, this antibody reacts with: heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins. Purity: > 95% based on SDS-PAGE.
Goat Anti-Mouse IgG (H+L) Antibody, Horseradish Peroxidase (HRP) Conjugate, is prepared from antibodies that have been adsorbed against bovine, horse, human, pig or rabbit serum proteins to minimize cross reactivity. Goat Anti-Mouse IgG (H+L) Antibody, Horseradish Peroxidase (HRP) Conjugate, should be suitable for western blot, ELISA, immunohistochemistry and other standard immunoassay applications. The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by isoelectric focusing (IEF).
Applications: Goat Anti-Mouse IgG (H+L) Antibody, Horseradish Peroxidase (HRP) Conjugate, should be suitable for western blot, ELISA, immunohistochemistry and other standard immunoassay applications.
Host species: The host species of theanti-mouse IgG (H+L), horseradish peroxidase (HRP) Conjugate, is goat.
Reactivity: Goat Anti-Mouse IgG (H+L) Antibody, Horseradish Peroxidase (HRP) Conjugate, detects mouse IgG (H+L).
Product size: 50μg
Suggested dilution: The optimal working dilution should be determined empirically. Dilutions of 1:500-1:5000 should be satisfactory for most immunohistochemical applications. Dilutions of 1:200-1:5,000 should be satisfactory for most ELISA and western blot applications.
Specifications
Specifications
| Applications | Immunoassay, Immunohistochemistry, Western Blot |
| Conjugate | HRP |
| Formulation | Lyophilized |
| Host Species | Goat |
| Purification Method | Affinity Purified |
| Quantity | 50 μg |
| Regulatory Status | RUO |
| Research Discipline | Protein Assays and Analysis |
| Whole Molecule | Whole Antibody |
| Primary or Secondary | Secondary |
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