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Invitrogen™ Goat anti-Human IgG1 (Heavy chain) Recombinant Secondary Antibody, Alexa Fluor™ 647

Product Code. 17866562
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50 μL
250 μL
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250µL
50µL
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17866562 250 μL 250µL
17837552 50 μL 50µL
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Product Code. 17866562 Supplier Invitrogen™ Supplier No. A55743

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Goat Recombinant Monoclonal Secondary Antibody

This Goat anti-Human IgG1 (Heavy chain) Recombinant Monoclonal Antibody specifically detects human IgG1 heavy chain with no cross-reactivity to human IgG2, IgG3, IgG4, IgA or IgM or various species immunoglobulins, including monkey IgG. The sensitivity and specificity of each lot is confirmed using ELISA. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Human IgG1 (Heavy chain)
Applications Flow Cytometry, Western Blot, Immunocytochemistry
Classification Recombinant Monoclonal
Clone 3H60L9
Concentration 1.0 mg/mL
Conjugate Alexa Fluor 647
Formulation PBS with 5mM sodium azide; pH 7.4
Host Species Goat
Immunogen Purified Human IgG.
Purification Method Protein G
Quantity 250 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Human
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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