Glucocorticoid Receptor Monoclonal Antibody (6E6), Invitrogen™

Product Code.	Quantity	unitSize
12847822	100 μL	100µL

Product Code. 12847822 Supplier Invitrogen Supplier No. MA515801

Description

Mouse Monoclonal Antibody

MA5-15801 targets NR3C1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15801 immunogen is purified recombinant fragment of human NR3C1 expressed in E. Coli. MA5-15801 detects NR3C1 which has a predicted molecular weight of approximately 86kDa.

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

Specifications

• Antigen: Glucocorticoid Receptor
• Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 6E6
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: ascites with 0.03% sodium azide
• Gene: Nr3c1
• Gene Accession No.: P04150, P06537
• Gene Alias: cytosolic/nuclear receptor; fb13f09; GCCR; Gcr; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Grl; Grl1; Grl-1; Nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858; utouto; utut; wu:fb13f09; zgc:113038
• Gene Symbols: Nr3c1
• Host Species: Mouse
• Immunogen: Purified recombinant fragment of human NR3C1 expressed in E. Coli.
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 14815, 2908
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1