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GFAP, Alexa Fluor 488, clone: GA5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-9892-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15342090

  • £76.64 / 25µg


EU Stock 5
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Description

Description

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistology staining of frozen and FFPE tissue sections, and immunocytochemistry.Applications Tested: This GA5 antibody has been tested by immunocytochemistry of fixed and permeabilized C6 cells and by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GFAP
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P47819, P14136, P03995
GFAP, FLJ45472
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Chicken, Human, Murine, Porcine, Rabbit, Rat
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
GA5
Alexa Fluor 488
GFAP
Liquid
GFAP
IgG1
25μg
4° C, store in dark, DO NOT FREEZE!
Primary
100352532, 14580, 24387, 2670, 396562, 419969
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For Research Use Only.