GFAP, Alexa Fluor 488, clone: GA5, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 53-9892-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistology staining of frozen and FFPE tissue sections, and immunocytochemistry.Applications Tested: This GA5 antibody has been tested by immunocytochemistry of fixed and permeabilized C6 cells and by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Glial Fibrillary Acid Protein (GFAP)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|P47819, P14136, P03995|
|Flow CytometryþImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)þMicroscopy|
|Alexa Fluor 488|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.