missing translation for 'onlineSavingsMsg'
Learn More

GFAP, Alexa Fluor 488, clone: GA5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-9892-80

Code : Z2

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15342090

  • £76.64 / 25µg


EU Stock 3
Add to basket

Description

Description

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistology staining of frozen and FFPE tissue sections, and immunocytochemistry.Applications Tested: This GA5 antibody has been tested by immunocytochemistry of fixed and permeabilized C6 cells and by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Glial Fibrillary Acid Protein (GFAP)
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P47819, P14136, P03995
GFAP, FLJ45472
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
ChickenþHumanþMurineþPorcineþRabbitþRat
Flow CytometryþImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)þMicroscopy
GA5
Alexa Fluor 488
GFAP
Conjugated
GFAP
IgG1
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
2670
Videos

For Research Use Only.