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GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
£56.50 - £216.00
Specifications
Antigen | GL7 |
---|---|
Clone | GL-7 (GL7) |
Concentration | 0.5 mg/mL |
Applications | Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15277587
|
Invitrogen
14-5902-81 |
50 μg |
£56.50
50µg |
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15287587
|
Invitrogen
14-5902-82 |
100 μg |
£82.50
100µg |
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15297587
|
Invitrogen
14-5902-85 |
500 μg |
£216.00
500µg |
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Description
Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.Specifications
GL7 | |
0.5 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with 0.09% sodium azide; pH 7.2 | |
IgM | |
Affinity chromatography | |
Antibody |
GL-7 (GL7) | |
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation | |
Unconjugated | |
Rat | |
Human, Mouse | |
GL7 antigen; Ly77; Ly-77 | |
Primary | |
4° C |
For Research Use Only.