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GILZ, clone: CFMKG15, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-4033-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15297347

  • £156.37 / 100µg


EU Stock 3
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Description

Description

Description: GILZ is a leucine-zipper-containing protein that is induced by glucocorticoids. It is expressed in mast cells, monocytes, macrophages, dendritic cells, and T cells. GILZ has been shown to inhibit production of IL-2 as well as TCR-driven upregulation of FasL. GILZ mediates these changes in transcription, at least in part, by inhibiting DNA-binding of the transcription factors AP-1 and NF-kappaB. GILZ has also been shown to mediate anti-inflammatory signals in macrophages and dendritic cells by interfering with NF-kappaB-mediated signaling. Although GILZ has been reported to localize to the nucleus, more recent reports as well as testing at eBioscience have observed predominantly cytoplasmic staining.Applications Reported: This CFMKG15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoblotting (WB), and immunocytochemistry. (Fluorochrome conjugated CFMKG15 is recommended for use in intracellular flow cytometry.).Applications Tested: This CFMKG15 antibody has been tested by western blot analysis of dexamethasone-treated mouse thymocytes. CFMKG15 recognizes endogenous mouse GILZ with an approximate molecular weight of 14 kDa; a non-specific band of approximately 95kDa is also observed. CFMKG15 also recognizes endogenous human GILZ in dexamethasone-treated peripheral blood mononuclear cells, with an approximate molecular weight of 14 kDa; non-specific bands may also appear at approximately 33kDa and 95 kDa. This can be used at less than or equal to 2 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GILZ
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q9Z2S7, Q99576
TSC22D3, TSC22 domain family member 3, TSC-22 related protein, delta sleep inducing peptide, DIP, DSIPI, GILZ, TSC-22R, DSIP-immunoreactive leucine zipper protein
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryþImmunocytochemistryþWestern Blot
CFMKG15
Unconjugated
TSC22D3
Purified
Tsc22d3
IgG2a, kappa
100μg
Store at 2-8°C.
Primary
1831
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