Gibco™ Versene Solution

Product Code.	Quantity	unitSize
12569069	100 mL	100mL

Product Code. 12569069 Supplier Gibco™ Supplier No. 15040066

Description

An EDTA solution for use as a gentle non-enzymatic cell dissociation reagent

• Gibco Versene Solution 0.48 mM
• Formulated as 0.2 g EDTA(Na₄) per liter of Phosphate Buffered Saline (PBS)
• Sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

• Chelators: EDTA
• Content And Storage: Storage conditions: 2 to 8°C
  Shipping conditions: Ambient
  Shelf life: 18 months from date of manufacture
• Classification: Animal Origin-free
• Product Type: Cell Culture Dissociation Reagent
• Sterility: Sterile-filtered
• With Additives: EDTA
• Manufacturing Quality: cGMP for medical devices, 21 CFR Part 820 and ISO 13485
• Quantity: 100 mL
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

Can PSCs transitioned over into Essential 8 Medium on recombinant human laminin-521 (rhLaminin-521) from feeder-dependent cultures, then be transferred onto vitronectin (VTN-N)?

Yes. Following 2 passages on the rhLaminin-521 matrix, Versene or EDTA passaging should be used to subculture PSCs.

What are the main differences to be taken into consideration when culturing cells in Gibco Essential 8 Medium on Gibco Vitronectin (VTN-N) compared to other feeder-free systems?

Here are three major differences to be taken into consideration when culturing cells in Gibco Essential 8 Medium on Gibco Vitronectin (VTN-N) compared to other feeder-free systems:
- Cells should be typically passaged ~24 hours sooner than they would be with other feeder-free media.
- Passaging should take place when cells are at ~85% confluency. If cells are passaged when they are more than 85% confluent, the health of the cells and final cell yield may be compromised.
- Cells must be passaged in EDTA. Collagenase and dispase are not recommended.

Can pluripotent stem cells (PSCs) previously cultured in other media and on other substrates be cultured in Gibco Essential 8 Medium and on Gibco Vitronectin?

Cells cultured in other feeder-free media systems, such as mTeSR Medium with Matrigel Basement Membrane Matrix, or StemPro hESC SFM with Geltrex Matrix, can be successfully cultured in Gibco Essential 8 Medium and VTN-N. In addition, PSCs grown on feeders with KnockOut SR have also been shown to be successfully cultured in Gibco Essential 8 Medium on VTN-N. However, when changing media systems, cells must be passaged either manually, or with EDTA prior to culturing in Gibco Essential 8 Medium on VTN-N.

What is the recommended passaging method to use with Gibco Essential 8 Medium and on Gibco Vitronectin (VTN-N)?

Cells cultured in Gibco Essential 8 Medium and VTN-N need to be passaged with EDTA.

I currently use mTeSR1. How difficult would it be to adapt my cells to the StemFlex Medium system?

This transition is very straightforward. We recommend at least a 2-passage transition into the StemFlex Medium system. Briefly, if you have cryopreserved cells previously cultured in the mTeSR1/Matrigel system, we recommend thawing the cells back into mTeSR1/Matrigel until fully recovered. Upon reaching ~70-85% confluency, passage using Versene solution and seed directly into the StemFlex Medium system.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Can I use EDTA or versene for passaging of stem cells?

Yes, you may use EDTA or versene. However, we do not recommend using dispase or collagenase as it can lead to differentiation.

Do I need to add a ROCK inhibitor during passaging when culturing in Gibco Essential 8 Flex Medium?

As with many other PSC media, ROCK inhibitor is not required with Essential 8 Flex Medium when Gibco Versene Solution is used to passage PSCs as clusters. If cells are plated as singlets, we suggest that Gibco RevitaCell Supplement (Cat. No A2644501) or another ROCK inhibitor be used.

Is Gibco Versene Solution still the recommended dissociation reagent with Gibco Essential 8 Flex Medium?

Both Versene Solution (Cat. No. 15040066) and enzyme-mediated dissociation have been shown to be compatible passaging methods.

For Research Use Only. Not for use in diagnostic procedures.