Gibco™ Trypsin-EDTA (0.5%), no phenol red

Product Code.	Quantity	unitSize
10779413	100 mL	100mL

Product Code. 10779413 Supplier Gibco™ Supplier No. 15400054

Description

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Due to its digestive strength, trypsin solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. Concentrated forms of trypsin can be aseptically diluted to 1X using a balanced salt solution without calcium and magnesium. The trypsin concentration required for dissociation varies with cell type and experimental requirements.

• Solutions tested for pH, osmolality, sterility, and performance
• Prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis
• Documented Traceability available including lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency

Sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Dry Ice

Compliance

Manufactured at a cGMP compliant facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Chelators: EDTA
• Content And Storage: Storage conditions: -5°C to -20°C
  
• Cell Type: Mammalian Cells
• Classification: Animal Origin
• Concentration: 10 X
• Form: Liquid
• Product Type: Cell Culture Dissociation Reagent
• Sterility: Sterile-filtered
• With Additives: EDTA
• Without Additives: No Phenol Red
• Osmolality: 280 - 340 mOsm/kg
• pH: 7.1 to 8.0
• Product Line: Gibco
• Quantity: 100 mL
• Shipping Condition: Wet Ice
• Source: Animal Origin
• Tested For: In vitro Bioassay

Frequently Asked Questions (FAQs)

I received Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054) with ice packs instead of dry ice and the product was partially melted. Is it OK to use?

Trypsin is shipped on wet ice as the shipment on dry ice can cause changes in pH which influences the product quality negatively. If you receive the trypsin thawed or partially thawed, the stability and functionality of the product will not be impacted. For further information please see the white paper at the following link.

What is the percentage of trypsin within the Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054 contains 0.5 % trypsin and 0.2% EDTA.

How do you recommend aliquoting Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend aliquoting the undiluted trypsin into single-use portions and storing the aliquots at -20 degrees C, so that fresh dilutions can be made whenever needed.

How many freeze/thaw cycles do you recommend for Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend keeping the freeze thaw cycles of trypsin to a minimum to avoid loss of enzyme activity.

What method do you recommend for thawing Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054)?

We recommend thawing Trypsin-EDTA (0.5%) at 4 degrees C overnight. Alternatively, you can thaw it in a 37 degrees C water bath, but you will need to keep a close eye on it because extended exposure at that temperature can cause a decrease in enzyme activity. If you use the 37 degrees C thawing method, we recommend swirling the bottle every 5 mins to allow the heat to distribute evenly. As soon as the trypsin is thawed, remove it from the water bath.

What trypsin concentration should be used to dissociate cells?

The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco Trypsin solutions and recommended dissociation conditions here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/trypsin.html).

What solution is the 10X Trypsin EDTA in?

It is in 8.5g/L NaCl.

What can I dilute my 10X Trypsin EDTA with?

Concentrated forms of trypsin can be aseptically diluted to 1X using a balanced salt solution without calcium and magnesium (eg. HBSS,Cat. No. 14170120).

Does Trypsin EDTA 0.5% solution (Cat. No. 15400xxx) contain Phenol Red?

No, this Trypsin EDTA solution is without Phenol Red

How long can I keep trypsin in the refrigerator after thawing?

Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Safety and Handling

Product Identifier

• TRYPSIN-EDTA 10X

Signal Word

• Danger

Hazard Category

• Respiratory sensitiser Category 1

Hazard Statement

• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P284-[In case of inadequate ventilation] wear respiratory protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contain: trypsin

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.