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Gibco™ RPMI 1640 Medium, no glutamine, no phenol red

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Brand:  Gibco™ 32404014

Code : 50

Additional Details : Weight : 0.50000kg

Product Code. 11564456

  • £15.83 / 500mL
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For Research Use Only. All usage must comply with product instructions.

Requires : Supplementation



Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:
Without: L-glutamine, HEPES, Phenol Red

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature


Gibco RPMI 1640 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.



Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Animal Origin-free
Standard Serum Supplementation
Sodium Bicarbonate
Sustainable packaging
260 - 300 mOsm/kg
500 mL
Leukemic Cells
1 X
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Room Temperature




Special Offers

Special Offers

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.