RPMI 1640 Medium, GlutaMAX™ Liquid Supplement, HEPES

Originally developed to culture human leukemic cells in suspension and as a monolayer

Brand: Gibco

Manufacturer Part Number: 72400047

500 ML RPMI MEDIUM 1640 (1X), LIQUID 500ML STOREin at 4°C in dark Shelf life 365 days

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 0.59000kg

Requires: Supplementation

Product Code. 11569726

Quantity Price
1 £20.91 / 500mL
EU Stock 7
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, HEPES, L-Alanyl-Glutamine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Quantity 500mL
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives No Sodium Pyruvate; GlutaMAX™-I; Phenol Red

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Modified as follows: with GlutaMAX™, phenol red, HEPES. RPMI 1640 medium with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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