Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Product Code.	Quantity	Unit Size
11569726	500 mL	500mL

Product Code. 11569726 Supplier Gibco™ Supplier No. 72400047

Beskrivning

Kräver

Supplementation

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension and as a monolayer.

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With: GlutaMAX™, Phenol Red, HEPES

The complete formulation is available.

Using RPMI 1640 Medium

RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B₁₂ and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Beställningsinfo

Shipping Condition: Room Temperature

Specifikationer

• Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
• Cell Type: Leukemic Cells
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: GlutaMAX, HEPES, Phenol Red
• Without Additives: No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco, GlutaMAX
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.