Gibco™ RPMI 1640 Medium (ATCC modification)

Originally developed to culture human leukemic cells in suspension and as a monolayer

Brand:  Gibco™ A1049101

Product Code. 11504566

Description

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

• Unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
• Contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
• Vitamins inositol and choline are present in high concentrations
• Modified by ATCC to contain high glucose, low sodium bicarbonate, sodium pyruvate, HEPES, low L-glutamine and phenol red
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses a sodium bicarbonate buffer system (1.5g/L) and therefore requires a 5-7% CO₂ environment to maintain physiological pH

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Compliance

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Content And Storage: Storage conditions: 2 – 8°C. Protect from light.
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• HEPES Buffer: HEPES
• Serum Supplementation: Standard Serum Supplementation
• Sodium Pyruvate Additive: Sodium Pyruvate
• Glutamine: L-Glutamine

• Glucose: High Glucose
• Product Line: Gibco®
• Shipping Condition: Room Temperature
• Form: Liquid
• Phenol Red Indicator: Phenol Red

For Research Use Only. Not for use in diagnostic procedures.