Opti-MEM™ Reduced Serum Medium, Powder

An improved Minimal Essential Medium that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology

Overview
£112.00 valid until 31/12/2016
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Brand: Gibco

Manufacturer Part Number: 22600134

10 LT OPTI MEM I, POWDER STORE AT 2-8C

UNSPSC: 12352207

Code: 50

Additional Details:
Additional Details: Weight: 0.14700kg



Product Code. 10134472

Quantity Price
1 £ 149.0 / Each
Estimated Shipment date
from Supplier 10-01-2017
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Format Powder
Quantity 10L
Storage Requirements Storage: 2-8° C. Shelf life: 30 months from date of manufacture
With Additives L-Glutamine; Phenol Red

Opti-MEM is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts.

  • Opti-MEM is modified as follows: with L-glutamine, phenol red
  • Unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements
  • These additional components allow for a reduction in serum supplementation by at least 50%
  • Uses sodium bicarbonate buffer system (2.4g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation
    • Cell Culture, Mammalian Cell Culture, Plasmid Transfection, RNAi Transfection, Synthetic siRNA Transfection, Transfection, shRNA and miR RNAi Plasmid Transfection

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