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Beschreibung
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I Reduced-Serum Medium modifications for a range of cell culture applications.
This Opti-MEM™ is modified as follows:
With: L-glutamine
Without: Phenol Red
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM™ Reduced-Serum Medium
Opti-MEM™ I medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Bestellinformation
Shipping Condition: Room Temperature
Compliance
Manufactured at a cGMP-compliant facility. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Spezifikation
Spezifikation
| Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
| Cell Type | Mammalian Cells |
| Classification | Human Origin |
| Concentration | 1X |
| Form | Liquid |
| Product Type | Opti-MEM I |
| Serum Level | Reduced Serum |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Glutamine |
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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