Gibco™ Sf-900™ II SFM, no methionine, no cystine

Product Code.	Quantity	Unit Size
11560546	500 mL	500mL

Product Code. 11560546 Supplier Gibco™ Supplier No. 21012026

Description

Optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells

Sf-900 II SFM (without methionine and cystine) is a serum-free, protein-free insect cell culture medium optimized for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium supports growth of other lepidopteran cell lines.

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Bioproduction, Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture, Viral Vaccine Production

Order Info

Shipping Condition: Room temperature

Especificaciones

• Cell Line: Sf21, Sf9
• Cell Type: Insect Cell
• Classification: Protein-free, Serum-free
• Form: Liquid
• Product Type: Insect Cell Serum Free Medium (SFM)
• Serum Level: Serum-free
• Product Line: Gibco, Sf-900
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Species: S. frugiperda, Spodoptera frugiperda
• Content And Storage: Storage conditions: 2°C to 8°C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

Frequently Asked Questions (FAQs)

What antimicrobials can be used in insect culture and at what concentration?

Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)

Is it necessary to heat-inactivate my serum before adding it to my medium?

Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.

Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.

Do you offer serum-free insect media?

Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.

For Research Use Only. Not for use in diagnostic procedures.