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Gibco™ MEM, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Brand:  Gibco™ 61100103

Code : 50

Additional Details : Weight : 0.47000kg

 View more versions of this product

Product Code. 11544516

  • £98.50 / 50 L
Estimated Shipment: 09-04-2024
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.

Using MEM
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Primary Rat Astrocytes
Powder
Standard Serum Supplementation
Low Glucose, Glutamine, Phenol Red
cGMP-compliant under the ISO 13485 standard
50 L
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Animal Origin-free
MEM (Minimum Essential Medium)
Sterile-filtered
No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
Gibco™
Room Temperature
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Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.